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This lecture supports students to get benefit from Geographical Information Systems (GIS) tools in water resources engineering. It introduces concepts of spatial coordinate systems, and raster and vector data types. The procedures of surface analysis using Digital Elevation Models (DEM) to the watershed delineation, including river networks are studied. In addition, the preparation of thematic data is introduced. Actually, this includes the sources of GIS input data from advanced on-site observations, Global Positioning System (GPS) and remote sensors. Then, different applications in ecological assessment of rivers, water supply and water management are reviewed. By the end of the lecture, a final project should be submitted by students applying GIS to address a specific topic of interest. Hands-on learning followed by theory introduction is expected.