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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variatio
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Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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The lethal poison Ricin (best known as a weapon of bioterrorism), Diphtheria toxin (the causative agent of a highly contagious bacterial disease), and the widely used antibiotic tetracycline have one thing in common: They specifically target the cell's translational apparatus and disrupt protein synthesis.
In this course, we will explore th
Author(s):
Tag(s):
- biology
- lethal poison
- ricin
- diphtheria
- contagious bacterial disease
- tetracycline
- protein synthesis
- drug resistance
- protein engineering
- cystic fibrosis
- muscular dystrophy
- ribosome
- ribosomal proteins
- rrna
- mrna
- trna
- translation factors
- genetic code
- e. coli ribosome
- prokaryotes
- eukaryotes
- shiga
- diphtheria toxin
- pseudomonas exotoxi
File: 7-344-spring-2007.zip
This OER is part of OCW: Antibiotics, Toxins, and Protein Engineering
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Inherited Tag(s):
- biology
- lethal poison
- ricin
- diphtheria
- contagious bacterial disease
- tetracycline
- protein synthesis
- drug resistance
- protein engineering
- cystic fibrosis
- muscular dystrophy
- ribosome
- ribosomal proteins
- rrna
- mrna
- trna
- translation factors
- genetic code
- e. coli ribosome
- prokaryotes
- eukaryotes
- shiga
- diphtheria toxin
- pseudomonas exotoxi
File: 7-13-fall-2008.zip
This OER is part of OCW: Experimental Microbial Genetics
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Used by:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Pages: 36
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Pages: 17
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: zpq8107.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: 10010983.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: zpq8101.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: pq004885.pdf
This OER is part of OCW: Experimental Microbial Genetics
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: jbacter00360-0204.pdf
This OER is part of OCW: Experimental Microbial Genetics
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: jb005990.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: 1770948.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: rna_footprinting.pdf
This OER is part of OCW: Antibiotics, Toxins, and Protein Engineering
Published under: /Readings
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OCW Authors:
Inherited Tag(s):
- biology
- lethal poison
- ricin
- diphtheria
- contagious bacterial disease
- tetracycline
- protein synthesis
- drug resistance
- protein engineering
- cystic fibrosis
- muscular dystrophy
- ribosome
- ribosomal proteins
- rrna
- mrna
- trna
- translation factors
- genetic code
- e. coli ribosome
- prokaryotes
- eukaryotes
- shiga
- diphtheria toxin
- pseudomonas exotoxi
File: primer_extension.pdf
This OER is part of OCW: Antibiotics, Toxins, and Protein Engineering
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- lethal poison
- ricin
- diphtheria
- contagious bacterial disease
- tetracycline
- protein synthesis
- drug resistance
- protein engineering
- cystic fibrosis
- muscular dystrophy
- ribosome
- ribosomal proteins
- rrna
- mrna
- trna
- translation factors
- genetic code
- e. coli ribosome
- prokaryotes
- eukaryotes
- shiga
- diphtheria toxin
- pseudomonas exotoxi
File: protein_engineer.pdf
This OER is part of OCW: Antibiotics, Toxins, and Protein Engineering
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- lethal poison
- ricin
- diphtheria
- contagious bacterial disease
- tetracycline
- protein synthesis
- drug resistance
- protein engineering
- cystic fibrosis
- muscular dystrophy
- ribosome
- ribosomal proteins
- rrna
- mrna
- trna
- translation factors
- genetic code
- e. coli ribosome
- prokaryotes
- eukaryotes
- shiga
- diphtheria toxin
- pseudomonas exotoxi
61 results found.
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