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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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This introductory biology laboratory course covers the application of experimental techniques in microbiology, biochemistry, cell and developmental biology. Emphasis is placed on the integration of factual knowledge with understanding of the design of the experiments and data analysis in order to prepare the students for future research pro
Author(s):
Tag(s):
- biology
- experimental biology
- microbial genetics
- protein biochemistry
- recombinant dna
- development
- zebrafish
- phase contrast microscopy
- teratogenesis
- rna isolation
- northern blot
- gene expression
- western blot
- pcr
- polymerase chain reaction
- rna gel
- rna fixation
- probe labeling
- mutagenesis
- transposon
- column chromatography
- size-exclusion
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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of re
Author(s):
Tag(s):
- biology
- microbiology
- genetics
- rhodococcus
- bacteria
- genes
- plasmid manipulation
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- gram-positive
- gram-negative
- bioconversion processes
- synthesis
- precursors
- metabolites
- genetic complementation
- laboratory
- lab
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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variatio
Author(s):
Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine wh
Author(s):
Tag(s):
- biology
- evolution
- biocatalyst
- mutation
- library
- recombination
- directed evolution
- enzyme
- point mutation
- mutagenesis
- dna
- gene
- complementation
- affinity
- phage
- ribosome display
- yeast surface display
- bacterial cell surface display
- ivc
- facs
- active site
File: 7-13-fall-2003.zip
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Download Course Materials
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Used by:
Inherited Tag(s):
- biology
- microbiology
- genetics
- rhodococcus
- bacteria
- genes
- plasmid manipulation
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- gram-positive
- gram-negative
- bioconversion processes
- synthesis
- precursors
- metabolites
- genetic complementation
- laboratory
- lab
File: 7-344-spring-2008.zip
This OER is part of OCW: Directed Evolution: Engineering Biocatalysts
Published under: /Download Course Materials
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Used by:
Inherited Tag(s):
- biology
- evolution
- biocatalyst
- mutation
- library
- recombination
- directed evolution
- enzyme
- point mutation
- mutagenesis
- dna
- gene
- complementation
- affinity
- phage
- ribosome display
- yeast surface display
- bacterial cell surface display
- ivc
- facs
- active site
File: 7-02-spring-2005.zip
This OER is part of OCW: Experimental Biology & Communication
Published under: /Download Course Materials
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Used by:
Inherited Tag(s):
- biology
- experimental biology
- microbial genetics
- protein biochemistry
- recombinant dna
- development
- zebrafish
- phase contrast microscopy
- teratogenesis
- rna isolation
- northern blot
- gene expression
- western blot
- pcr
- polymerase chain reaction
- rna gel
- rna fixation
- probe labeling
- mutagenesis
- transposon
- column chromatography
- size-exclusion
File: 7-13-fall-2008.zip
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Download Course Materials
Share in:
Used by:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Pages: 36
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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Pages: 17
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Assignments
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: zpq8107.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: 10010983.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: zpq8101.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: pq004885.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: jbacter00360-0204.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
File: jb005990.pdf
This OER is part of OCW: Experimental Microbial Genetics
Published under: /Readings
Share in:
OCW Authors:
Inherited Tag(s):
- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
208 results found.
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