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Published by: Massachusetts Institute of Technology | Language: English
Published by: Massachusetts Institute of Technology | Language: English
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In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variatio
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- biology
- microbiology
- genetics
- pseudomonas
- bacteria
- genes
- pathogen
- mutagenesis
- pcr
- dna sequencing
- enzyme assays
- gene expression
- molecular genetics
- plasmid manipulation
- genetic complementation
- laboratory
- protocol
- vector
- mutant
- cystic fibrosis
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